I am new here and in the bioinformatics world, please, be kind(:

I am currently trying to implement a pipeline using SingleCellExperiment and DropletUtils in R. I have been getting an error for while now and I have tired everything to fix it, but nothing works. This is the code I a, currently running.

I’m trying to develop an eDNA metabarcoding assay and I’m searching using the r package primerTree to search universal fish primer pairs. When I search I don’t want to search for all organisms that have a sequence that works. I want to search only within a specific genus. Does anyone know how to do this?